Plasmid

Part:BBa_K5181017:Design

Designed by: Mayukh Mitra   Group: iGEM24_IISER-TVM   (2024-09-24)


pRGPDuo3_CPR_P450


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4543
    Illegal XbaI site found at 152
    Illegal SpeI site found at 3137
    Illegal PstI site found at 140
    Illegal PstI site found at 461
    Illegal PstI site found at 1139
    Illegal PstI site found at 3811
    Illegal PstI site found at 4090
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4543
    Illegal NheI site found at 3391
    Illegal SpeI site found at 3137
    Illegal PstI site found at 140
    Illegal PstI site found at 461
    Illegal PstI site found at 1139
    Illegal PstI site found at 3811
    Illegal PstI site found at 4090
    Illegal NotI site found at 5494
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4543
    Illegal BglII site found at 4427
    Illegal BglII site found at 6065
    Illegal BamHI site found at 158
    Illegal XhoI site found at 1550
    Illegal XhoI site found at 1696
    Illegal XhoI site found at 5700
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4543
    Illegal XbaI site found at 152
    Illegal SpeI site found at 3137
    Illegal PstI site found at 140
    Illegal PstI site found at 461
    Illegal PstI site found at 1139
    Illegal PstI site found at 3811
    Illegal PstI site found at 4090
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4543
    Illegal XbaI site found at 152
    Illegal SpeI site found at 3137
    Illegal PstI site found at 140
    Illegal PstI site found at 461
    Illegal PstI site found at 1139
    Illegal PstI site found at 3811
    Illegal PstI site found at 4090
    Illegal NgoMIV site found at 590
    Illegal NgoMIV site found at 5429
    Illegal NgoMIV site found at 7101
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

later


Source

later

References